OsDCL1a activation impairs phytoalexin biosynthesis and compromises disease resistance in rice.
Identifieur interne : 000422 ( Main/Exploration ); précédent : 000421; suivant : 000423OsDCL1a activation impairs phytoalexin biosynthesis and compromises disease resistance in rice.
Auteurs : Raquel Salvador-Guirao [Espagne] ; Patricia Baldrich [Espagne, États-Unis] ; Shiho Tomiyama [Japon] ; Yue-Ie Hsing [Taïwan] ; Kazunori Okada [Japon] ; Blanca San Segundo [Espagne]Source :
- Annals of botany [ 1095-8290 ] ; 2019.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Maladies des plantes, Oryza, Protéines végétales.
- métabolisme : Oryza, Protéines végétales, Sesquiterpènes.
- physiologie : Magnaporthe.
- Résistance à la maladie.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Plant Proteins.
- genetics : Oryza, Plant Diseases.
- metabolism : Oryza, Plant Proteins, Sesquiterpenes.
- physiology : Magnaporthe.
- Disease Resistance.
Abstract
Background and Aims
MicroRNAs (miRNAs) are small non-coding RNAs that act as post-transcriptional regulators of gene expression via sequence-specific cleavage or translational repression of target transcripts. They are transcribed as long single-stranded RNA precursors with unique stem-loop structures that are processed by a DICER-Like (DCL) ribonuclease, typically DCL1, to produce mature miRNAs. Although a plethora of miRNAs have been found to be regulated by pathogen infection in plants, the biological function of most miRNAs remains largely unknown. Here, the contribution of OsDCL1 to rice immunity was investigated.
Methods
Activation-tagged Osdcl1a (Osdcl1a-Ac) rice mutants were examined for resistance to pathogen infection. mRNA and small RNA deep sequencing, quantitative real-time PCR (RT-qPCR) and stem-loop reverse tanscripion-PCR (RT-PCR) were used to examine DCL1a-mediated alterations in the rice transcriptome. Rice diterpene phytoalexins were quantified by liquid chromatography-tandem mass spectrometry (LC-MSMS). Accumulation of O2·- was determined by nitroblue tetrazolium (NBT) staining.
Key Results
dcl1a-Ac mutants exhibit enhanced susceptibility to infection by fungal pathogens which was associated with a weaker induction of defence gene expression. Comparison of the mRNA and miRNA transcriptomes of dcl1a-Ac and wild-type plants revealed misregulation of genes involved in detoxification of reactive oxygen species. Consequently, dcl1a-Ac plants accumulated O2·- in their leaves and were more sensitive to methyl viologen-induced oxidative stress. Furthermore, dcl1a-Ac plants showed downregulation of diterpenoid phytoalexin biosynthetic genes, these genes also being weakly induced during pathogen infection. Upon pathogen challenge, dcl1a-Ac plants failed to accumulate major diterpenoid phytoalexins. OsDCL1a activation resulted in marked alterations in the rice miRNAome, including both upregulation and downregulation of miRNAs.
Conclusions
OsDCL1a activation enhances susceptibility to infection by fungal pathogens in rice. Activation of OsDCL1a represses the pathogen-inducible host defence response and negatively regulates diterpenoid phytoalexin production. These findings provide a basis to understand the molecular mechanisms through which OsDCL1a mediates rice immunity.
DOI: 10.1093/aob/mcy141
PubMed: 30032201
PubMed Central: PMC6344094
Affiliations:
- Espagne, Japon, Taïwan, États-Unis
- Catalogne, Missouri (État), Région de Kantō
- Barcelone, Tokyo
- Université de Tokyo
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">OsDCL1a activation impairs phytoalexin biosynthesis and compromises disease resistance in rice.</title><author><name sortKey="Salvador Guirao, Raquel" sort="Salvador Guirao, Raquel" uniqKey="Salvador Guirao R" first="Raquel" last="Salvador-Guirao">Raquel Salvador-Guirao</name><affiliation wicri:level="3"><nlm:affiliation>Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus Universitat Autònoma de Barcelona (UAB), Bellaterra (Cerdanyola del Vallés), Barcelona, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus Universitat Autònoma de Barcelona (UAB), Bellaterra (Cerdanyola del Vallés), Barcelona</wicri:regionArea><placeName><settlement type="city">Barcelone</settlement><region nuts="2" type="region">Catalogne</region></placeName></affiliation></author><author><name sortKey="Baldrich, Patricia" sort="Baldrich, Patricia" uniqKey="Baldrich P" first="Patricia" last="Baldrich">Patricia Baldrich</name><affiliation wicri:level="3"><nlm:affiliation>Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus Universitat Autònoma de Barcelona (UAB), Bellaterra (Cerdanyola del Vallés), Barcelona, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus Universitat Autònoma de Barcelona (UAB), Bellaterra (Cerdanyola del Vallés), Barcelona</wicri:regionArea><placeName><settlement type="city">Barcelone</settlement><region nuts="2" type="region">Catalogne</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>Donald Danforth Plant Science Center, St Louis, MO, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Donald Danforth Plant Science Center, St Louis, MO</wicri:regionArea><placeName><region type="state">Missouri (État)</region></placeName></affiliation></author><author><name sortKey="Tomiyama, Shiho" sort="Tomiyama, Shiho" uniqKey="Tomiyama S" first="Shiho" last="Tomiyama">Shiho Tomiyama</name><affiliation wicri:level="4"><nlm:affiliation>Biotechnology Research Center, The University of Tokyo, Tokyo, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Biotechnology Research Center, The University of Tokyo, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName></affiliation></author><author><name sortKey="Hsing, Yue Ie" sort="Hsing, Yue Ie" uniqKey="Hsing Y" first="Yue-Ie" last="Hsing">Yue-Ie Hsing</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Institute of Plant and Microbial Biology, Academia Sinica, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Okada, Kazunori" sort="Okada, Kazunori" uniqKey="Okada K" first="Kazunori" last="Okada">Kazunori Okada</name><affiliation wicri:level="4"><nlm:affiliation>Biotechnology Research Center, The University of Tokyo, Tokyo, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Biotechnology Research Center, The University of Tokyo, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName></affiliation></author><author><name sortKey="San Segundo, Blanca" sort="San Segundo, Blanca" uniqKey="San Segundo B" first="Blanca" last="San Segundo">Blanca San Segundo</name><affiliation wicri:level="3"><nlm:affiliation>Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus Universitat Autònoma de Barcelona (UAB), Bellaterra (Cerdanyola del Vallés), Barcelona, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus Universitat Autònoma de Barcelona (UAB), Bellaterra (Cerdanyola del Vallés), Barcelona</wicri:regionArea><placeName><settlement type="city">Barcelone</settlement><region nuts="2" type="region">Catalogne</region></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Consejo Superior de Investigaciones Científicas (CSIC), Barcelona, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Consejo Superior de Investigaciones Científicas (CSIC), Barcelona</wicri:regionArea><placeName><settlement type="city">Barcelone</settlement><region nuts="2" type="region">Catalogne</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:30032201</idno><idno type="pmid">30032201</idno><idno type="doi">10.1093/aob/mcy141</idno><idno type="pmc">PMC6344094</idno><idno type="wicri:Area/Main/Corpus">000760</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000760</idno><idno type="wicri:Area/Main/Curation">000760</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000760</idno><idno type="wicri:Area/Main/Exploration">000760</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">OsDCL1a activation impairs phytoalexin biosynthesis and compromises disease resistance in rice.</title><author><name sortKey="Salvador Guirao, Raquel" sort="Salvador Guirao, Raquel" uniqKey="Salvador Guirao R" first="Raquel" last="Salvador-Guirao">Raquel Salvador-Guirao</name><affiliation wicri:level="3"><nlm:affiliation>Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus Universitat Autònoma de Barcelona (UAB), Bellaterra (Cerdanyola del Vallés), Barcelona, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus Universitat Autònoma de Barcelona (UAB), Bellaterra (Cerdanyola del Vallés), Barcelona</wicri:regionArea><placeName><settlement type="city">Barcelone</settlement><region nuts="2" type="region">Catalogne</region></placeName></affiliation></author><author><name sortKey="Baldrich, Patricia" sort="Baldrich, Patricia" uniqKey="Baldrich P" first="Patricia" last="Baldrich">Patricia Baldrich</name><affiliation wicri:level="3"><nlm:affiliation>Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus Universitat Autònoma de Barcelona (UAB), Bellaterra (Cerdanyola del Vallés), Barcelona, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus Universitat Autònoma de Barcelona (UAB), Bellaterra (Cerdanyola del Vallés), Barcelona</wicri:regionArea><placeName><settlement type="city">Barcelone</settlement><region nuts="2" type="region">Catalogne</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>Donald Danforth Plant Science Center, St Louis, MO, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Donald Danforth Plant Science Center, St Louis, MO</wicri:regionArea><placeName><region type="state">Missouri (État)</region></placeName></affiliation></author><author><name sortKey="Tomiyama, Shiho" sort="Tomiyama, Shiho" uniqKey="Tomiyama S" first="Shiho" last="Tomiyama">Shiho Tomiyama</name><affiliation wicri:level="4"><nlm:affiliation>Biotechnology Research Center, The University of Tokyo, Tokyo, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Biotechnology Research Center, The University of Tokyo, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName></affiliation></author><author><name sortKey="Hsing, Yue Ie" sort="Hsing, Yue Ie" uniqKey="Hsing Y" first="Yue-Ie" last="Hsing">Yue-Ie Hsing</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Institute of Plant and Microbial Biology, Academia Sinica, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Okada, Kazunori" sort="Okada, Kazunori" uniqKey="Okada K" first="Kazunori" last="Okada">Kazunori Okada</name><affiliation wicri:level="4"><nlm:affiliation>Biotechnology Research Center, The University of Tokyo, Tokyo, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Biotechnology Research Center, The University of Tokyo, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName></affiliation></author><author><name sortKey="San Segundo, Blanca" sort="San Segundo, Blanca" uniqKey="San Segundo B" first="Blanca" last="San Segundo">Blanca San Segundo</name><affiliation wicri:level="3"><nlm:affiliation>Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus Universitat Autònoma de Barcelona (UAB), Bellaterra (Cerdanyola del Vallés), Barcelona, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus Universitat Autònoma de Barcelona (UAB), Bellaterra (Cerdanyola del Vallés), Barcelona</wicri:regionArea><placeName><settlement type="city">Barcelone</settlement><region nuts="2" type="region">Catalogne</region></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Consejo Superior de Investigaciones Científicas (CSIC), Barcelona, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Consejo Superior de Investigaciones Científicas (CSIC), Barcelona</wicri:regionArea><placeName><settlement type="city">Barcelone</settlement><region nuts="2" type="region">Catalogne</region></placeName></affiliation></author></analytic><series><title level="j">Annals of botany</title><idno type="eISSN">1095-8290</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Disease Resistance (MeSH)</term><term>Magnaporthe (physiology)</term><term>Oryza (genetics)</term><term>Oryza (metabolism)</term><term>Plant Diseases (genetics)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Sesquiterpenes (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Magnaporthe (physiologie)</term><term>Maladies des plantes (génétique)</term><term>Oryza (génétique)</term><term>Oryza (métabolisme)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Résistance à la maladie (MeSH)</term><term>Sesquiterpènes (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Oryza</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Maladies des plantes</term><term>Oryza</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Oryza</term><term>Plant Proteins</term><term>Sesquiterpenes</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Oryza</term><term>Protéines végétales</term><term>Sesquiterpènes</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Magnaporthe</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Magnaporthe</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Disease Resistance</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Résistance à la maladie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>Background and Aims</b></p><p>MicroRNAs (miRNAs) are small non-coding RNAs that act as post-transcriptional regulators of gene expression via sequence-specific cleavage or translational repression of target transcripts. They are transcribed as long single-stranded RNA precursors with unique stem-loop structures that are processed by a DICER-Like (DCL) ribonuclease, typically DCL1, to produce mature miRNAs. Although a plethora of miRNAs have been found to be regulated by pathogen infection in plants, the biological function of most miRNAs remains largely unknown. Here, the contribution of OsDCL1 to rice immunity was investigated.</p></div><div type="abstract" xml:lang="en"><p><b>Methods</b></p><p>Activation-tagged Osdcl1a (Osdcl1a-Ac) rice mutants were examined for resistance to pathogen infection. mRNA and small RNA deep sequencing, quantitative real-time PCR (RT-qPCR) and stem-loop reverse tanscripion-PCR (RT-PCR) were used to examine DCL1a-mediated alterations in the rice transcriptome. Rice diterpene phytoalexins were quantified by liquid chromatography-tandem mass spectrometry (LC-MSMS). Accumulation of O2·- was determined by nitroblue tetrazolium (NBT) staining.</p></div><div type="abstract" xml:lang="en"><p><b>Key Results</b></p><p>dcl1a-Ac mutants exhibit enhanced susceptibility to infection by fungal pathogens which was associated with a weaker induction of defence gene expression. Comparison of the mRNA and miRNA transcriptomes of dcl1a-Ac and wild-type plants revealed misregulation of genes involved in detoxification of reactive oxygen species. Consequently, dcl1a-Ac plants accumulated O2·- in their leaves and were more sensitive to methyl viologen-induced oxidative stress. Furthermore, dcl1a-Ac plants showed downregulation of diterpenoid phytoalexin biosynthetic genes, these genes also being weakly induced during pathogen infection. Upon pathogen challenge, dcl1a-Ac plants failed to accumulate major diterpenoid phytoalexins. OsDCL1a activation resulted in marked alterations in the rice miRNAome, including both upregulation and downregulation of miRNAs.</p></div><div type="abstract" xml:lang="en"><p><b>Conclusions</b></p><p>OsDCL1a activation enhances susceptibility to infection by fungal pathogens in rice. Activation of OsDCL1a represses the pathogen-inducible host defence response and negatively regulates diterpenoid phytoalexin production. These findings provide a basis to understand the molecular mechanisms through which OsDCL1a mediates rice immunity.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30032201</PMID><DateCompleted><Year>2019</Year><Month>12</Month><Day>30</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>06</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1095-8290</ISSN><JournalIssue CitedMedium="Internet"><Volume>123</Volume><Issue>1</Issue><PubDate><Year>2019</Year><Month>01</Month><Day>01</Day></PubDate></JournalIssue><Title>Annals of botany</Title><ISOAbbreviation>Ann Bot</ISOAbbreviation></Journal><ArticleTitle>OsDCL1a activation impairs phytoalexin biosynthesis and compromises disease resistance in rice.</ArticleTitle><Pagination><MedlinePgn>79-93</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/aob/mcy141</ELocationID><Abstract><AbstractText Label="Background and Aims">MicroRNAs (miRNAs) are small non-coding RNAs that act as post-transcriptional regulators of gene expression via sequence-specific cleavage or translational repression of target transcripts. They are transcribed as long single-stranded RNA precursors with unique stem-loop structures that are processed by a DICER-Like (DCL) ribonuclease, typically DCL1, to produce mature miRNAs. Although a plethora of miRNAs have been found to be regulated by pathogen infection in plants, the biological function of most miRNAs remains largely unknown. Here, the contribution of OsDCL1 to rice immunity was investigated.</AbstractText><AbstractText Label="Methods">Activation-tagged Osdcl1a (Osdcl1a-Ac) rice mutants were examined for resistance to pathogen infection. mRNA and small RNA deep sequencing, quantitative real-time PCR (RT-qPCR) and stem-loop reverse tanscripion-PCR (RT-PCR) were used to examine DCL1a-mediated alterations in the rice transcriptome. Rice diterpene phytoalexins were quantified by liquid chromatography-tandem mass spectrometry (LC-MSMS). Accumulation of O2·- was determined by nitroblue tetrazolium (NBT) staining.</AbstractText><AbstractText Label="Key Results">dcl1a-Ac mutants exhibit enhanced susceptibility to infection by fungal pathogens which was associated with a weaker induction of defence gene expression. Comparison of the mRNA and miRNA transcriptomes of dcl1a-Ac and wild-type plants revealed misregulation of genes involved in detoxification of reactive oxygen species. Consequently, dcl1a-Ac plants accumulated O2·- in their leaves and were more sensitive to methyl viologen-induced oxidative stress. Furthermore, dcl1a-Ac plants showed downregulation of diterpenoid phytoalexin biosynthetic genes, these genes also being weakly induced during pathogen infection. Upon pathogen challenge, dcl1a-Ac plants failed to accumulate major diterpenoid phytoalexins. OsDCL1a activation resulted in marked alterations in the rice miRNAome, including both upregulation and downregulation of miRNAs.</AbstractText><AbstractText Label="Conclusions">OsDCL1a activation enhances susceptibility to infection by fungal pathogens in rice. Activation of OsDCL1a represses the pathogen-inducible host defence response and negatively regulates diterpenoid phytoalexin production. These findings provide a basis to understand the molecular mechanisms through which OsDCL1a mediates rice immunity.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Salvador-Guirao</LastName><ForeName>Raquel</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus Universitat Autònoma de Barcelona (UAB), Bellaterra (Cerdanyola del Vallés), Barcelona, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Baldrich</LastName><ForeName>Patricia</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus Universitat Autònoma de Barcelona (UAB), Bellaterra (Cerdanyola del Vallés), Barcelona, Spain.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Donald Danforth Plant Science Center, St Louis, MO, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tomiyama</LastName><ForeName>Shiho</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Biotechnology Research Center, The University of Tokyo, Tokyo, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hsing</LastName><ForeName>Yue-Ie</ForeName><Initials>YI</Initials><AffiliationInfo><Affiliation>Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Okada</LastName><ForeName>Kazunori</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Biotechnology Research Center, The University of Tokyo, Tokyo, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>San Segundo</LastName><ForeName>Blanca</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus Universitat Autònoma de Barcelona (UAB), Bellaterra (Cerdanyola del Vallés), Barcelona, Spain.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Consejo Superior de Investigaciones Científicas (CSIC), Barcelona, Spain.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>T37 TW000041</GrantID><Acronym>TW</Acronym><Agency>FIC NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Ann Bot</MedlineTA><NlmUniqueID>0372347</NlmUniqueID><ISSNLinking>0305-7364</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012717">Sesquiterpenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>37297-20-4</RegistryNumber><NameOfSubstance UI="C011991">phytoalexins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D060467" MajorTopicYN="N">Disease Resistance</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020082" MajorTopicYN="N">Magnaporthe</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012275" MajorTopicYN="N">Oryza</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012717" MajorTopicYN="N">Sesquiterpenes</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>04</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>06</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>7</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>12</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>7</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30032201</ArticleId><ArticleId IdType="pii">5055718</ArticleId><ArticleId IdType="doi">10.1093/aob/mcy141</ArticleId><ArticleId IdType="pmc">PMC6344094</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Biosci Biotechnol Biochem. 2013;77(4):760-5</Citation><ArticleIdList><ArticleId IdType="pubmed">23563545</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2008 Oct 01;9:451</Citation><ArticleIdList><ArticleId IdType="pubmed">18826656</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2002 Apr 5;296(5565):92-100</Citation><ArticleIdList><ArticleId IdType="pubmed">11935018</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2013;8(3):e59720</Citation><ArticleIdList><ArticleId IdType="pubmed">23544090</ArticleId></ArticleIdList></Reference><Reference><Citation>Biosci Biotechnol Biochem. 2003 Apr;67(4):899-902</Citation><ArticleIdList><ArticleId IdType="pubmed">12784637</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Physiol. 1996 Jan;37(1):9-18</Citation><ArticleIdList><ArticleId IdType="pubmed">8720923</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 2009 Feb;69(3):337-46</Citation><ArticleIdList><ArticleId IdType="pubmed">19039666</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Sci. 2015 Aug;237:24-32</Citation><ArticleIdList><ArticleId IdType="pubmed">26089149</ArticleId></ArticleIdList></Reference><Reference><Citation>Mycobiology. 2013 Dec;41(4):225-33</Citation><ArticleIdList><ArticleId IdType="pubmed">24493944</ArticleId></ArticleIdList></Reference><Reference><Citation>FEBS Lett. 2006 May 1;580(10):2442-50</Citation><ArticleIdList><ArticleId IdType="pubmed">16638569</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2012;7(12):e51609</Citation><ArticleIdList><ArticleId IdType="pubmed">23251593</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2012 Feb;69(3):462-74</Citation><ArticleIdList><ArticleId IdType="pubmed">21973320</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Phytopathol. 2011;49:317-43</Citation><ArticleIdList><ArticleId IdType="pubmed">21663438</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 2005 Dec 29;123(7):1279-91</Citation><ArticleIdList><ArticleId IdType="pubmed">16377568</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2014 Apr 1;30(7):923-30</Citation><ArticleIdList><ArticleId IdType="pubmed">24227677</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2012 May;40(10):4288-97</Citation><ArticleIdList><ArticleId IdType="pubmed">22287627</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2014 Feb;164(2):1077-92</Citation><ArticleIdList><ArticleId IdType="pubmed">24335508</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2014 Mar 11;111(10):3877-82</Citation><ArticleIdList><ArticleId IdType="pubmed">24554078</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 2004 Aug 18;23(16):3356-64</Citation><ArticleIdList><ArticleId IdType="pubmed">15282547</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2014 Aug 1;30(15):2114-20</Citation><ArticleIdList><ArticleId IdType="pubmed">24695404</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2016 Mar;29(3):165-9</Citation><ArticleIdList><ArticleId IdType="pubmed">26867095</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Plant Sci. 2017 Jan 17;8:2</Citation><ArticleIdList><ArticleId IdType="pubmed">28144248</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Plant Sci. 2011 May;16(5):258-64</Citation><ArticleIdList><ArticleId IdType="pubmed">21466971</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Gen Genet. 1992 Jul;234(1):97-104</Citation><ArticleIdList><ArticleId IdType="pubmed">1495490</ArticleId></ArticleIdList></Reference><Reference><Citation>Environ Microbiol. 2010 Mar;12(3):649-57</Citation><ArticleIdList><ArticleId IdType="pubmed">20002135</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2013 Jun;26(6):617-25</Citation><ArticleIdList><ArticleId IdType="pubmed">23489060</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Biotechnol J. 2008 Aug;6(6):585-608</Citation><ArticleIdList><ArticleId IdType="pubmed">18444970</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2013 Sep 1;29(17):2146-52</Citation><ArticleIdList><ArticleId IdType="pubmed">23821648</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2014 Aug;79(4):659-78</Citation><ArticleIdList><ArticleId IdType="pubmed">24450747</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2009 May;150(1):320-32</Citation><ArticleIdList><ArticleId IdType="pubmed">19321708</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Plant Biol. 2013 May;16(2):170-9</Citation><ArticleIdList><ArticleId IdType="pubmed">23466255</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Phytopathol. 2014;52:495-516</Citation><ArticleIdList><ArticleId IdType="pubmed">25090478</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2016 Aug;87(3):293-304</Citation><ArticleIdList><ArticleId IdType="pubmed">27133567</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2010 Aug;23(8):1000-11</Citation><ArticleIdList><ArticleId IdType="pubmed">20615111</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Biol. 2005 Aug 23;15(16):1494-500</Citation><ArticleIdList><ArticleId IdType="pubmed">16040244</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2013 Jul;199(1):212-27</Citation><ArticleIdList><ArticleId IdType="pubmed">23627500</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 2009 Mar;229(4):1009-14</Citation><ArticleIdList><ArticleId IdType="pubmed">19148671</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2005 Sep;139(1):296-305</Citation><ArticleIdList><ArticleId IdType="pubmed">16126864</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2008 May 30;320(5880):1185-90</Citation><ArticleIdList><ArticleId IdType="pubmed">18483398</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2008 Aug 15;321(5891):964-7</Citation><ArticleIdList><ArticleId IdType="pubmed">18703740</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2013 Jan 1;29(1):15-21</Citation><ArticleIdList><ArticleId IdType="pubmed">23104886</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2003 Sep 18;425(6955):257-63</Citation><ArticleIdList><ArticleId IdType="pubmed">12931144</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2002 Apr 5;296(5565):79-92</Citation><ArticleIdList><ArticleId IdType="pubmed">11935017</ArticleId></ArticleIdList></Reference><Reference><Citation>RNA Biol. 2015;12(8):847-63</Citation><ArticleIdList><ArticleId IdType="pubmed">26083154</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Microbiol. 2009 Mar;7(3):185-95</Citation><ArticleIdList><ArticleId IdType="pubmed">19219052</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Immunol. 2016 Sep;16(9):537-52</Citation><ArticleIdList><ArticleId IdType="pubmed">27477127</ArticleId></ArticleIdList></Reference><Reference><Citation>Biosci Biotechnol Biochem. 2013;77(6):1141-8</Citation><ArticleIdList><ArticleId IdType="pubmed">23748776</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2006 Nov 16;444(7117):323-9</Citation><ArticleIdList><ArticleId IdType="pubmed">17108957</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 2007 Feb;63(3):351-64</Citation><ArticleIdList><ArticleId IdType="pubmed">17120135</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Plant Sci. 2012 Feb;17(2):73-90</Citation><ArticleIdList><ArticleId IdType="pubmed">22209038</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2010 Apr;152(4):2222-31</Citation><ArticleIdList><ArticleId IdType="pubmed">20164210</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytopathology. 2002 Aug;92(8):870-6</Citation><ArticleIdList><ArticleId IdType="pubmed">18942966</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2002 Sep 20;297(5589):2053-6</Citation><ArticleIdList><ArticleId IdType="pubmed">12242443</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Rep. 2016 May 06;6:25493</Citation><ArticleIdList><ArticleId IdType="pubmed">27150822</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cell. 2006 Apr 7;22(1):129-36</Citation><ArticleIdList><ArticleId IdType="pubmed">16600876</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15849-54</Citation><ArticleIdList><ArticleId IdType="pubmed">9861059</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2006 Nov 21;103(47):18002-7</Citation><ArticleIdList><ArticleId IdType="pubmed">17071740</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2004 Aug 24;101(34):12753-8</Citation><ArticleIdList><ArticleId IdType="pubmed">15314213</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2006 Apr 21;312(5772):436-9</Citation><ArticleIdList><ArticleId IdType="pubmed">16627744</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2010 Jul;38(Web Server issue):W64-70</Citation><ArticleIdList><ArticleId IdType="pubmed">20435677</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Espagne</li><li>Japon</li><li>Taïwan</li><li>États-Unis</li></country><region><li>Catalogne</li><li>Missouri (État)</li><li>Région de Kantō</li></region><settlement><li>Barcelone</li><li>Tokyo</li></settlement><orgName><li>Université de Tokyo</li></orgName></list><tree><country name="Espagne"><region name="Catalogne"><name sortKey="Salvador Guirao, Raquel" sort="Salvador Guirao, Raquel" uniqKey="Salvador Guirao R" first="Raquel" last="Salvador-Guirao">Raquel Salvador-Guirao</name></region><name sortKey="Baldrich, Patricia" sort="Baldrich, Patricia" uniqKey="Baldrich P" first="Patricia" last="Baldrich">Patricia Baldrich</name><name sortKey="San Segundo, Blanca" sort="San Segundo, Blanca" uniqKey="San Segundo B" first="Blanca" last="San Segundo">Blanca San Segundo</name><name sortKey="San Segundo, Blanca" sort="San Segundo, Blanca" uniqKey="San Segundo B" first="Blanca" last="San Segundo">Blanca San Segundo</name></country><country name="États-Unis"><region name="Missouri (État)"><name sortKey="Baldrich, Patricia" sort="Baldrich, Patricia" uniqKey="Baldrich P" first="Patricia" last="Baldrich">Patricia Baldrich</name></region></country><country name="Japon"><region name="Région de Kantō"><name sortKey="Tomiyama, Shiho" sort="Tomiyama, Shiho" uniqKey="Tomiyama S" first="Shiho" last="Tomiyama">Shiho Tomiyama</name></region><name sortKey="Okada, Kazunori" sort="Okada, Kazunori" uniqKey="Okada K" first="Kazunori" last="Okada">Kazunori Okada</name></country><country name="Taïwan"><noRegion><name sortKey="Hsing, Yue Ie" sort="Hsing, Yue Ie" uniqKey="Hsing Y" first="Yue-Ie" last="Hsing">Yue-Ie Hsing</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/DetoxFungiV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000422 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000422 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= DetoxFungiV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:30032201
|texte= OsDCL1a activation impairs phytoalexin biosynthesis and compromises disease resistance in rice.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:30032201" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a DetoxFungiV1
| This area was generated with Dilib version V0.6.38. |  |